Peer reviewed


Hurley, M.E., Sheard, T.M.D., Norman, R., Kirton, H.M., Shah, S.S., Pervolaraki, E., Yang, Z., Gamper, N., White, E., Steele, D. & Jayasinghe, I. A correlative super-resolution protocol to visualise structural underpinnings of fast second-messenger signalling in primary cell types. Methods doi: 10.1016/j.ymeth.2020.10.005

Colman, M.A., Holmes, M., Whittaker, D., Jayasinghe, I., Benson, A. Multi-scale approaches for the simulation of cardiac electrophysiology: I – sub-cellular and stochastic calcium dynamics from cell to organ. Methods. S1046-2023(19)30225-7


Sheard, T.M.D., Hurley, M.E., Colyer, J., White, E., Norman, R., Pervolaraki, E., Narayanasamy, K.K., Hou, Y., Kirton, H., Yang, Z., Hunter, L., Shim, J., Clowsley, A.H., Smith, A.J., Baddeley, D., Soeller, C., Colman, M.A., Jayasinghe, I. Three-Dimensional and Chemical Mapping of Intracellular Signalling Nanodomains in Health and Disease with Enhanced Expansion Microscopy. ACS Nano. 2019. DOI: 10.1021/acsnano.8b08742

Miteva, K.T.,  Pedicini, L., Wilson, L.A., Jayasinghe, I. , Slip, R.G., Marszalek, K., Gaunt, H.J., Bartoli, F., Deivasigamani, S., Sobradillo, D., Beech, D.J. and McKeown, L. Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies. J Cell Biol. 2019 DOI: 10.1083/jcb.201810118


Jayasinghe, I., Clowsley, A.H., Green, E., Harrison, C., Lutz, T.,Baddeley, D., di Michele, L., Soeller, C. True molecular scale visualisation of variable clustering properties of ryanodine receptors. Cell Reports. 2018; 22 (2), 557-567

Jayasinghe, I., Clowsley, A.H., Sali, S., Crossman, D.J., Soeller, C. Shining new light on the structural determinants of cardiac couplon function. Frontiers in Physiology. doi: 10.3389/fphys.2018.01472


Lin, R., Clowsley, A.H., Jayasinghe, I.D., Baddeley, D., Soeller, C.. Algorithmic corrections for localization microscopy with sCMOS cameras-characterisation of a computationally efficient localization approach. Optics Express. 2017; 25 (10), 11701-11716

Crossman, D.J., Jayasinghe, I.D. Soeller, C. T-tubule remodelling: a cellular pathology driven by both sides of the plasmalemma. Biophysical Reviews. 2017. doi:10.1007/s12551-017-0273-7.

Cully, T.R., Murphy, R.M., Roberts, L., Raastad, T., Fassett, R., Coombes, J.S., Jayasinghe, I.D., Launikonis, B.S. The plasmalemma of human skeletal muscle alters its structure to change its Ca2+ handling in response to heavy-load resistance exercise. Nature Communications. 2017; 8: 14266


Munro, M.L., Jayasinghe, I.D., Wang, Q., Quick, A., Wang, W., Baddeley, D., Wehrens, X.H.T., Soeller, C. The role of Junctophilin-2 in the nanoscale organisation and functional gating of ryanodine receptor clusters in murine cardiomyocytes. J Cell Sci. 2016; 29(23):4388-4398


*Hou, Y., *Jayasinghe, I.D., Crossman, D.J., Baddeley, D., Soeller, C. Nanoscale analysis of ryanodine receptor clusters in dyadic couplings of rat cardiac myocytes. JMCC. 2015; 80 (1): 45-55.* Equal contributors

Crossman, D.J., Hou, Y., Jayasinghe, I.D., Baddeley, D., Soeller, C. Combining confocal and single molecule localisation microscopy: A correlative approach to multi-scale tissue imaging. Methods. 2015; 88, 98-108

Jayasinghe, I.D., Clowsley, A., Hou, Y., Munro, M., Crossman, D.J., Soeller, C. Revealing t-tubules in striated muscle with new optical super-resolution microscopy techniques. Eur J TransMyol. 2015; 25 (1): 15-26.


Jayasinghe, I.D., Munro, M., Baddeley, D., Launikonis, B.S., Soeller, C. Observation of the molecular organization of calcium release sites in fast- and slow-twitch skeletal muscle with nanoscale imaging. J. R. Soc. Interface. 2014. 11(99) pii: 20140570

Hou, Y., Crossman, D.J. …  Jayasinghe, I.D., Soeller, C. Super-resolution fluorescence imaging to study cardiac biophysics: α-actinin distribution and z-disk topologies in optically thick cardiac tissue slices. Prog. Biophys. Mol. Biol.. 2014. 115 (2-3): 328–339.


Jayasinghe I.D., Launikonis B.S. Three-dimensional reconstruction and analysis of the tubular system of vertebrate skeletal muscle. J Cell Sci. 2013. 126(17). 4048-58.

Jayasinghe I.D., Lo H.P., Morgan G.P., Baddeley D., Parton R.G., Soeller C., Launikonis B. Examination of the sub-sarcolemmal tubular system of mammalian skeletal muscle fibres. Biophys J. 2013. 104 (11): L-19-2


Jayasinghe I.D., Baddeley D., Kong C.H-T., Wehrens, X.T., Cannell M.B., Soeller C.. Nanoscale organization of junctophilin-2 and ryanodine receptors within peripheral couplings of rat ventricular cardiomyocytes. Biophys J. 2012. 102 (5): L19-21.

Jayasinghe I.D., Crossman D.J., Soeller C., Cannell M.B. Comparison of the organization of t-tubules, sarcoplasmic reticulum and ryanodine receptors in rat and human ventricular myocardium Clin Exp Pharmacol Physiol. 2012. 39 (5):469-76.

2009 – 2011

Baddeley, D., Crossman, D.J., Rossberger, S., Cheyne, J., Montgomery, J., Jayasinghe, I.D., Cremer, C., Cannell, M.B., Soeller, C. 4D super-resolution microscopy with conventional fluorophores and single wavelength excitation in optically thick cells and tissues. PLoS One 2011; 6(5): e20645.

Jayasinghe, I.D., Crossman, D.J., Soeller, C., Cannell, M.B. A new twist in cardiac muscle: dislocated and helicoid arrangements of myofibrillar z-disks in mammalian ventricular myocytes. J Mol Cell Cardiol. 2010; 48 (5): 964-971.

Baddeley, D., Jayasinghe, I.D., Lam, L., Rossberger, S., Cannell, M.B., Soeller, C. Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes. Proc Natl Acad Sci U S A. 2009; 106 (52): 22275-80.

Jayasinghe, I.D., Cannell, M.B., Soeller, C. Organization of ryanodine receptors, transverse tubules and sodium-calcium exchanger in rat myocytes. Biophys J 2009; 97: 2664-73.

Baddeley, D., Jayasinghe, I.D.., Cremer, C., Cannell, M. B., Soeller, C. Light-induced dark states of organic fluochromes enable 30 nm resolution imaging in standard media. Biophys J. 2009, 96, L22-24.

Soeller, C., Jayasinghe, I.D., Li, P., Holden, A., Cannell, M.B. Three-dimensional high-resolution imaging of cardiac proteins to construct models of intracellular Ca2+ signalling in rat ventricular myocytes. Exp Physiol. 2009; 94 (5): 496-508.

Other articles

Lin R., Clowsley A., Jayasinghe I.D., Soeller C.. Single-Molecule Localization Microscopy with sCMOS Cameras. June 2015 Biophotonics.

Book chapters

Sheard, T.M.D and Jayasinghe, I., Enhanced expansion microscopy to measure nanoscale structural and biochemical remodeling in single cells; In Methods in Cell Biology; vol 161, 2020 DOI:10.1016/bs.mcb.2020.04.019

Jayasinghe, I., Clowsley, A. & Soeller, C. Advances in the visualisation of molecular assemblies within cellular signalling nanodomains: insights from a decade of mapping of ryanodine receptor clusters; In Advances in Biomembranes and Lipid Self-Assembly; vol 28. 2018

Soeller, C. & Jayasinghe, I.D. Quantitative super-resolution microscopy of cardiomyocytes. In press in Cardiac Imaging series by Springer Publishing group; edited by Lars Kaestner.

Soeller, C., Hou, Y., Jayasinghe I.D., Baddeley, D., Crossman, D.. Correlative single molecule localisation microscopy and confocal microscopy. In press “Super-resolution microscopy: methods & protocols: Methods in Molecular Biology series” by Springer Sciences.