UK Research & Innovation Future Leader Fellowship awarded to develop a compact super-resolution microscopy technology

The principal investigator of the Nanoscale Microscopy Group, Dr Izzy Jayasinghe, has been awarded a UKRI Future Leader Fellowship to build a new portable imaging technology which will allow scientists, medical doctors, conservationists and industrial parties to visualise the smallest building blocks of any biological sample from any location. https://www.youtube.com/watch?v=vqkzDOiWVXs/ The UK and other governments …

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New paper on “Enhanced Expansion Microscopy” and application on 3D and chemical in situ mapping of RyR

We demonstrate the ability to exploit in-plane resolution of ~ 15 nm and axial resolution of ~ 35 nm by combining X10 Expansion Microscopy with Airyscan 3D imaging. Expansion Microscopy is the newest of super-resolution imaging methods which allows finer details of samples to be visualised with relatively conventional fluorescence imaging techniques by physically expanding …

Tom Sheard reporting on doctoral research placement at the University of Auckland, New Zealand

In October 2018 I flew across the world to the laboratory of Dr David Crossman at the University of Auckland in New Zealand. The goal: to study pathological remodelling in human heart biopsies from patients with idiopathic dilated cardiomyopathy (IDCM), using the super-resolution imaging technique expansion microscopy (ExM). Our interest lies in seeing whether nanodomain …

New review out now: Shining New Light on the Structural Determinants of Cardiac Couplon Function

Our new review article to mark 10 years since the first super-resolution imaging experiments on cardiac muscle:  Abstract: Remodelling of the membranes and protein clustering patterns during the pathogenesis of cardiomyopathies has renewed the interest in spatial visualisation of these structures in cardiomyocytes. Coincidental emergence of single molecule (super-resolution) imaging and tomographic electron microscopy tools in the …

New review chapter: “Advances in the Visualization of Molecular Assemblies Within Cellular Signaling Nanodomains: Insights From a Decade of Mapping of Ryanodine Receptor Clusters”

Nanodomains are naturally assembled signaling stations, which facilitate fast and highly regulated signaling within and between cells. Calcium (Ca2+) nanodomains known as junctional membrane complexes (JMCs) transduce fast and highly synchronized intracellular signals, which are required by a variety of cell types. Common to most such nanodomains are clustered assemblies of the principal intracellular Ca2+ release channels, …